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Home >> Antibodies >> VWF Antibody / von Willebrand factor

VWF Antibody / von Willebrand factor [clone MSVA-521R] (V6130)

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  Catalog No Formulation Size Price (USD)  
Image V6130-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6130-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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von Willebrand factor Rabbit Recombinant Monoclonal Antibody (MSVA-521R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-521R
UniProt P04275
Localization Extracellular matrix, Extracellular space, Secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This VWF/von Willebrand factor antibody is available for research use only.
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Application Notes

1. Optimal dilution of the VWF/von Willebrand factor antibody should be determined by the researcher.

2. This VWF/von Willebrand factor antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

A recombinant fragment of human vWF protein (around amino acids 1815-1939) (exact sequence is proprietary) was used as the immuongen for the VWF/von Willebrand factor antibody.

Storage

VWF/von Willebrand factor antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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