Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody [clone VM452] (V3935)

Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody Raji Cell WB. Western blot analysis of Raji cell lysate using Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody. A distinct immunoreactive band is detected at approximately 53-58 kDa, consistent with the expected molecular weight of vimentin, supporting specific detection of the endogenous type III intermediate filament protein in Raji cells.

Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody Human Melanoma IHC. Immunohistochemical staining of FFPE human melanoma tissue using Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody. Strong cytoplasmic vimentin expression is observed throughout the melanoma cells, consistent with the mesenchymal phenotype and widespread expression of this type III intermediate filament protein in melanocytic tumors. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 10–20 minutes, followed by cooling at room temperature for 20 minutes prior to immunostaining.

Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody Jurkat Cell FACS. Flow cytometric analysis of Jurkat cells using Alexa Fluor 488-conjugated Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody. The green histogram shows a clear rightward shift relative to the isotype control (gray) and unstained cells (black), demonstrating specific detection of endogenous vimentin expression by flow cytometry.

Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody Protein Microarray Validation. Analysis of the HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins using Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody demonstrates exceptional target specificity. Clone VM452 produced a strong signal for VIM with a Z-score of 119.35 and an S-score of 104.76, supporting highly selective recognition of vimentin with minimal cross-reactivity. The Z-score reflects the strength of antibody binding relative to the array background, while the S-score represents the specificity of the antibody for its intended target compared with the next highest-ranking proteins.

SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone VM452) as confirmation of integrity and purity.

Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody U-87 MG Cell WB. Western blot analysis of U-87 MG cell lysate using Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody. A distinct immunoreactive band is detected at approximately 53-58 kDa, consistent with the expected molecular weight of vimentin, supporting specific detection of the endogenous type III intermediate filament protein in U-87 MG cells.

Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody A375 Cell WB. Western blot analysis of A375 cell lysate using Vimentin Antibody Clone VM452 / VIM Mesenchymal Marker Antibody. A distinct immunoreactive band is detected at approximately 53-58 kDa, consistent with the expected molecular weight of vimentin, supporting specific detection of the endogenous type III intermediate filament protein in A375 melanoma cells.