Vimentin Antibody [clone VM452] (V3935)

Western blot testing of Vimentin antibody and Raji cell lysate. Expected molecular weight: 53-58 kDa.

IHC staining of FFPE human melanoma with Vimentin antibody (clone VM452). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of Jurkat cells and Alexa Fluor 488 conjugated Vimentin antibody (green), cells alone (black) and isotype control (gray).

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Vimentin antibody (clone VM452). These results demonstrate the foremost specificity of the VM452 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone VM452) as confirmation of integrity and purity.

Western blot testing of Vimentin antibody and U-87 MG cell lysate. Expected molecular weight: 53-58 kDa.

Western blot testing of Vimentin antibody and A375 cell lysate. Expected molecular weight: 53-58 kDa.