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Home >> Antibodies >> VEGFA Antibody / Vascular Endothelial Growth Factor A

VEGFA Antibody / Vascular Endothelial Growth Factor A [clone VEGF/9366] (V5806)

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  Catalog No Formulation Size Price (USD)  
Image V5806-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V5806-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V5806SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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IHC staining of FFPE human placenta tissue with VEGFA antibody (clone VEGF/9366). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free VEGFA antibody (clone VEGF/9366) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2, kappa
Clone Name VEGF/9366
Purity Protein G affinity
UniProt P15692
Localization Cytoplasmic, cell surface and extracellular (secreted)
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This VEGFA antibody is available for research use only.
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Description

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

For additional information about VEGF biology, validated applications, and related products, learn more about our VEGF Antibody, an Angiogenesis Growth Factor Antibody for investigating endothelial signaling, tumor angiogenesis, vascular remodeling, and VEGF-mediated growth factor pathways.

Application Notes

Optimal dilution of the VEGFA antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1-200 from human VEGF protein was used as the immunogen for the VEGFA antibody.

Storage

Aliquot the VEGFA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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