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Home >> Antibodies >> Vascular Endothelial Growth Factor A Antibody / VEGFA

Vascular Endothelial Growth Factor A Antibody / VEGFA [clone VEGF/9368] (V5807)

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  Catalog No Formulation Size Price (USD)  
Image V5807-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5807-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5807SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human placenta tissue with VEGFA antibody (clone VEGF/9368). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placenta tissue with VEGFA antibody (clone VEGF/9368). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free VEGFA antibody (clone VEGF/9368) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2, kappa
Clone Name VEGF/9368
Purity Protein G affinity
UniProt P15692
Localization Cytoplasmic, cell surface and extracellular (secreted)
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Vascular Endothelial Growth Factor A antibody is available for research use only.
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Description

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Application Notes

Optimal dilution of the Vascular Endothelial Growth Factor A antibody should be determined by the researcher.

Immunogen

Human VEGF189 recombinant protein was used as the immunogen for the Vascular Endothelial Growth Factor A antibody.

Storage

Aliquot the Vascular Endothelial Growth Factor A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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