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Home >> Antibodies >> UCHL3 Antibody

UCHL3 Antibody [clone 26U65] (RQ8920)

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Image RQ8920 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 439
Microvalidated Recrabbitmono
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Immunofluorescent staining of FFPE human U-2 OS cells with UCHL3 antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with UCHL3 antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human K562, 2) human 293T, 3) human A431, 4) human Caco-2, 5) rat heart and 6) mouse heart tissue lysate with UCHL3 antibody. Predicted molecular weight ~26 kDa.
Availability 1-2 weeks
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name 26U65
Purity Affinity purified
UniProt P15374
Localization Nucleus, Cytoplasm, Golgi
Applications Western Blot : 1:500
Immunofluorescence : 1:50
Limitations This UCHL3 antibody is available for research use only.
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Description

UCHL3 (Ubiquitin carboxyl-terminal hydrolase isozyme L3) is a deubiquitinating enzyme that belongs to the ubiquitin C-terminal hydrolase family. It functions by processing ubiquitin precursors and recycling ubiquitin from ubiquitinated proteins, thereby maintaining cellular ubiquitin homeostasis. Researchers often use a UCHL3 antibody to explore its role in protein turnover and cellular quality control.

UCHL3 is widely expressed across tissues and has been linked to key biological processes including DNA repair, cell cycle regulation, and stress responses. Dysregulation of UCHL3 has been associated with cancer, neurodegeneration, and impaired protein degradation pathways. Employing a UCHL3 antibody provides insights into its diverse cellular functions and disease associations.

NSJ Bioreagents offers a high-quality UCHL3 antibody validated for applications such as western blot, immunohistochemistry, and immunoprecipitation. Using a UCHL3 antibody ensures sensitive and reproducible detection in studies of ubiquitin biology, proteostasis, and disease mechanisms.

Application Notes

Optimal dilution of the UCHL3 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human UCHL3 was used as the immunogen for the UCHL3 antibody.

Storage

Store the UCHL3 antibody at -20oC.

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