Tyrosinase Antibody / Melanin Biosynthesis Enzyme Antibody [clone TYR/3826] (V9597)

Tyrosinase Antibody Human Skin IHC. Immunohistochemical staining of FFPE human skin using Tyrosinase antibody (clone TYR/3826) demonstrated specific cytoplasmic staining of melanocytes within the basal layer of the epidermis, consistent with the restricted expression pattern of tyrosinase in pigment-producing cells. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to staining. The primary antibody was used at 2 ug/ml. This Tyrosinase Antibody, also called a Melanin Biosynthesis Enzyme Antibody, is useful for investigating melanocyte biology, melanin biosynthesis, pigmentation disorders, and melanoma research.

Tyrosinase Antibody Human Melanoma IHC. Immunohistochemical staining of FFPE human melanoma tissue using Tyrosinase antibody (clone TYR/3826) demonstrated strong cytoplasmic staining in malignant melanocytic cells, consistent with the role of tyrosinase as the rate-limiting enzyme in melanin biosynthesis. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to staining. The primary antibody was used at 2 ug/ml. This Tyrosinase Antibody, also called a Melanin Biosynthesis Enzyme Antibody, is useful for investigating melanocyte biology, melanin biosynthesis, pigmentation disorders, and melanoma research.

Tyrosinase Antibody Protein Microarray Validated. Analysis of the HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins demonstrated exceptional specificity of Tyrosinase antibody (clone TYR/3826). TYR was identified as the top-ranked target with a Z-score of 108.13 and an S-score of 100.22, while the next highest-ranking proteins (ERBB2 and FSCB) showed negligible S-scores, indicating minimal off-target binding. The Z-score represents the binding signal strength relative to the mean signal across the array, expressed as standard deviations above the mean. The S-score represents the difference in Z-scores between adjacent ranked proteins, with larger values indicating greater target specificity. These data independently validate the outstanding specificity of clone TYR/3826 for studies of melanocyte biology, melanin biosynthesis, pigmentation disorders, and melanoma research.