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Home >> Antibodies >> Tspan1 Antibody

Tspan1 Antibody (RQ6215)

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  Catalog No Formulation Size Price (USD)  
Image RQ6215 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE rat stomach tissue with Tspan1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE rat stomach tissue with Tspan1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of rat small intestine with Tspan1 antibody. Expected molecular weight: 28-30 kDa.
Flow cytometry testing of rat RH35 cells with Tspan1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tspan1 antibody.
IHC staining of FFPE rat stomach tissue with Tspan1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.0125% sodium azide
UniProt Q6AYR9
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Immunofluorescence : 5ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This Tspan1 antibody is available for research use only.
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Description

TSPAN1 belongs to the large family of tetraspanins, so named because they contain 4 transmembrane domains. Tetraspanins are frequently expressed at the cell surface in association with each other and with other molecules, such as integrins, and they function to regulate cell adhesion, migration, proliferation, and differentiation.

Application Notes

Optimal dilution of the Tspan1 antibody should be determined by the researcher.

Immunogen

A rat recombinant partial protein (amino acids A125-A212) was used as the immunogen for the Tspan1 antibody.

Storage

After reconstitution, the Tspan1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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