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Home >> Antibodies >> TOP2A Antibody / DNA topoisomerase II alpha

TOP2A Antibody / DNA topoisomerase II alpha [clone MSVA-802R] (V6123)

  Catalog No Formulation Size Price (USD)  
Image V6123-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 709.50
Image
V6123-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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DNA topoisomerase 2-alpha Rabbit Recombinant Monoclonal Antibody (MSVA-802R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-802R
UniProt P11388
Localization Cytoplasm, Nucleolus, Nucleoplasm, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This TOP2A/DNA topoisomerase II alpha antibody is available for research use only.
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Application Notes

1. Optimal dilution of the TOP2A/DNA topoisomerase II alpha antibody should be determined by the researcher.

2. This TOP2A/DNA topoisomerase II alpha antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary) was used as the immuongen for the TOP2A/DNA topoisomerase II alpha antibody.

Storage

TOP2A/DNA topoisomerase II alpha antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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