TLE1 Antibody Microarray Specificity Validated Clone TLE1/2085 / Transducin-like enhancer protein 1 [clone TLE1/2085] (V3926)

TLE1 Antibody Microarray Specificity Validated Clone TLE1/2085 western blot analysis of Transducin-Like Enhancer Protein 1 / TLE1. Lane 1: human HeLa cell lysate. A band is detected at approximately 83 kDa, consistent with the predicted molecular weight of Transducin-Like Enhancer Protein 1 / TLE1. Clone TLE1/2085 is a Microarray Specificity Validated antibody, indicating antibody selectivity was evaluated using proteome-scale protein microarray screening to assess binding across thousands of individual proteins. The detected band corresponds to endogenous TLE1 protein expressed in human HeLa cells and is consistent with the nuclear transcriptional corepressor role of TLE1 within Groucho family transcriptional repression complexes.

TLE1 Antibody Microarray Specificity Validated Clone TLE1/2085 immunohistochemistry analysis of human endometrial carcinoma. Formalin-fixed paraffin-embedded human endometrial carcinoma tissue shows strong nuclear staining in tumor epithelial cells consistent with expression of Transducin-Like Enhancer Protein 1, a nuclear transcriptional corepressor belonging to the Groucho/TLE family of transcriptional regulators. Clone TLE1/2085 is a Microarray Specificity Validated antibody, indicating antibody selectivity was assessed using proteome-scale protein microarray screening to evaluate binding across thousands of individual proteins and confirm preferential recognition of the intended target protein. Nuclear staining highlights malignant epithelial cells while surrounding stromal components show weaker or minimal signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 10-20 minutes followed by cooling at room temperature prior to antibody staining.

TLE1 Antibody Microarray Specificity Validated Clone TLE1/2085 protein microarray specificity analysis. Proteome-scale screening was performed using a HuProt human protein microarray containing more than 19,000 individually purified full-length human proteins. The TLE1 Antibody Microarray Specificity Validated Clone TLE1/2085 demonstrated highly selective binding to Transducin-Like Enhancer Protein 1, producing the strongest signal on the array with a Z-score of 130.01 and an S-score of 116.26, indicating strong enrichment for the intended target relative to all other proteins tested. Secondary signals detected for EFTUD2 and PARL were markedly lower, confirming the antibody's strong preferential recognition of TLE1. These results support the designation of clone TLE1/2085 as a Microarray Specificity Validated antibody, demonstrating proteome-wide selectivity through independent screening against thousands of human proteins to evaluate antibody specificity at a global scale.
Z-score represents the strength of the fluorescent signal generated when the antibody binds to a protein on the array, expressed as standard deviations above the mean signal intensity across the entire microarray. S-score represents the difference between sequential Z-scores after ranking targets by signal intensity, providing a quantitative measure of relative specificity for the intended antigen compared with other proteins present on the array.

SDS-PAGE analysis of purified, BSA-free TLE1 antibody (clone TLE1/2085) as confirmation of integrity and purity.