TLE1 Antibody Protein Microarray Validated Clone TLE1/2062 / Transducin-like enhancer protein 1 [clone TLE1/2062] (V3925)

TLE1 Antibody Protein Microarray Validated Clone TLE1/2062 immunohistochemistry analysis of human gastrointestinal stromal tumor (GIST). Formalin-fixed paraffin-embedded human GIST tissue shows nuclear staining in tumor cells consistent with expression of Transducin-Like Enhancer Protein 1, a nuclear transcriptional corepressor belonging to the Groucho/TLE family of transcriptional regulators. Clone TLE1/2062 is a protein microarray validated antibody, indicating specificity assessment using large-scale protein microarray screening to evaluate antibody binding across extensive protein panels. Nuclear staining highlights tumor cells within the gastrointestinal stromal tumor while surrounding stromal elements show weaker or absent signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 10-20 minutes followed by cooling at room temperature prior to antibody staining.

TLE1 Antibody Protein Microarray Validated Clone TLE1/2062 immunohistochemistry analysis of human endometrial carcinoma. Formalin-fixed paraffin-embedded human endometrial carcinoma tissue shows strong nuclear staining in tumor cells consistent with expression of Transducin-Like Enhancer Protein 1, a nuclear transcriptional corepressor belonging to the Groucho/TLE family of transcriptional regulators. Clone TLE1/2062 is a Protein Microarray Validated antibody, indicating antibody specificity was evaluated using large-scale protein microarray screening across extensive protein panels. Nuclear staining highlights malignant epithelial cells while surrounding stromal components show weaker or minimal signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 10-20 minutes followed by cooling at room temperature prior to antibody staining.

TLE1 Antibody Protein Microarray Validated Clone TLE1/2062 western blot analysis of Transducin-Like Enhancer Protein 1 / TLE1. Lane 1: human HeLa cell lysate. A band is detected at approximately 83 kDa, consistent with the predicted molecular weight of Transducin-Like Enhancer Protein 1 / TLE1. Clone TLE1/2062 is a Protein Microarray Validated antibody, indicating specificity was evaluated using large-scale protein microarray screening across extensive protein panels to assess selective recognition of the intended target protein. The detected band corresponds to endogenous TLE1 protein expressed in human HeLa cells, consistent with the nuclear transcriptional corepressor role of TLE1 within Groucho family transcriptional repression complexes.

TLE1 Antibody Protein Microarray Validated Clone TLE1/2062 specificity analysis using HuProt human protein microarray. A comprehensive human protein microarray containing more than 19,000 full-length human proteins was probed with TLE1 Antibody Protein Microarray Validated Clone TLE1/2062 to evaluate antibody specificity across a large proteome-scale panel. The results demonstrate strong and selective binding of the antibody to Transducin-Like Enhancer Protein 1 (TLE1), with TLE1 producing the highest signal intensity among all proteins present on the array. This microarray validation confirms that Clone TLE1/2062 shows preferential recognition of the intended target protein compared with other proteins screened in the assay.
The Z-score represents the strength of signal generated when the antibody binds to a specific protein on the HuProt microarray and is expressed in units of standard deviations above the mean signal across the array. Proteins are ranked according to Z-score values, with higher scores indicating stronger antibody binding. The S-score represents the difference between the Z-scores of sequentially ranked proteins and reflects the relative specificity of the antibody for its intended target. A large S-score between the top-ranked protein and the next highest signal indicates strong target specificity. In this analysis, Transducin-Like Enhancer Protein 1 produced the highest Z-score and a strong S-score separation from other proteins, demonstrating the high specificity of TLE1 Antibody Protein Microarray Validated Clone TLE1/2062 in proteome-scale screening.

SDS-PAGE analysis of purified, BSA-free TLE1 antibody (clone TLE1/2062) as confirmation of integrity and purity.