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Home >> Antibodies >> TLE1 Antibody / Transducin-like enhancer protein 1

TLE1 Antibody / Transducin-like enhancer protein 1 [clone TLE1/2085] (V3926)

  Catalog No Formulation Size Price (USD)  
Image V3926-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3926-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3926SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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Western blot testing of human HeLa cell lysate with TLE1 antibody (clone TLE1/2085). Predicted molecular weight ~83 kDa.
IHC testing of FFPE human endometrial carcinoma with TLE1 antibody (clone TLE1/2085). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TLE1 antibody (clone TLE1/2085). These results demonstrate the foremost specificity of the TLE1/2085 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free TLE1 antibody (clone TLE1/2085) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name TLE1/2085
Purity Protein G affinity chromatography
UniProt Q04724
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This TLE1 antibody is available for research use only.
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Description

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Application Notes

Optimal dilution of the TLE1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 175-338 from the human protein was used as the immunogen for the TLE1 antibody.

Storage

Store the TLE1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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