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Home >> Antibodies >> Survivin Antibody

Survivin Antibody (R32641)

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  Catalog No Formulation Size Price (USD)  
Image R32641 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat PC-12, 2) human K562 and 3) mouse thymus cell lysate with Survivin antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
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Western blot testing of 1) rat PC-12, 2) human K562 and 3) mouse thymus cell lysate with Survivin antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
Western blot testing of human 293T cell lysate with Survivin antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
IHC staining of FFPE human intestinal cancer with Survivin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with Survivin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt O15392
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
ELISA : 0.1-0.5ug/ml (human protein tested; request BSA-free format for coating)
Limitations This Survivin antibody is available for research use only.
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Description

Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. It is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions as inhibitor caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. And this protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.

Application Notes

Optimal dilution of the Survivin antibody should be determined by the researcher.

Immunogen

Amino acids M1-D142 from the human protein were used as the immunogen for the Survivin antibody.

Storage

After reconstitution, the Survivin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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