STAT6 Antibody [clone STAT6/2410] (V3893)

Immunohistochemistry analysis of STAT3 antibody in human renal cell carcinoma tissue (clone STAT6/2410). FFPE human renal cell carcinoma sections demonstrate HRP-DAB brown cytoplasmic and nuclear staining in tumor cells forming glandular and tubular structures. The staining pattern highlights both cytoplasmic localization and nuclear accumulation consistent with activated Signal transducer and activator of transcription 3 in neoplastic epithelial cells. Surrounding stromal components show minimal background staining. Heat induced epitope retrieval was performed in pH 6, 10 mM citrate buffer by boiling tissue sections for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using STAT6 antibody (clone STAT6/2410). These results demonstrate the foremost specificity of the STAT6/2410 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free STAT6 antibody (clone STAT6/2410) as confirmation of integrity and purity.

IHC staining of FFPE human liposarcoma tissue with STAT3 antibody (clone STAT6/2410). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry staining of PFA-fixed human HeLa cells with STAT6 antibody; Red=isotype control, Blue= STAT6 antibody.