StAR Antibody Microarray Specificity Validated Clone STAR/2154 / Steroidogenic acute regulatory protein [clone STAR/2154] (V3897)

StAR Antibody Microarray Specificity Validated Clone STAR/2154. Immunohistochemistry analysis of Steroidogenic Acute Regulatory Protein (StAR / STARD1) in FFPE human adrenal gland tissue using a mouse monoclonal antibody validated by large-scale protein microarray specificity screening. HRP-DAB brown cytoplasmic staining highlights steroidogenic cells of the adrenal cortex, consistent with the mitochondrial localization of StAR in steroid hormone-producing endocrine cells, while surrounding non-steroidogenic tissues show minimal staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris with 1 mM EDTA) for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody staining.

StAR Antibody Microarray Specificity Validated Clone STAR/2154. Immunohistochemistry analysis of Steroidogenic Acute Regulatory Protein (StAR / STARD1) in FFPE human testicular carcinoma tissue using a mouse monoclonal antibody validated by large-scale protein microarray specificity screening. HRP-DAB brown cytoplasmic staining highlights tumor cells with steroidogenic differentiation, consistent with the mitochondrial localization of StAR in steroid hormone-producing cells, while surrounding stromal and non-steroidogenic tissues show minimal staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris with 1 mM EDTA) for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to staining.

IHC staining of FFPE human testicular carcinoma with StAR antibody (clone STAR/2154). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

StAR Antibody Microarray Specificity Validated Clone STAR/2154. Protein microarray specificity validation using a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective recognition of Steroidogenic Acute Regulatory Protein (StAR / STARD1) by the STAR/2154 mouse monoclonal antibody. Signal ranking analysis shows STARD1 as the top target with a Z-score of 134.64 and S-score of 127.37, while all other proteins display substantially lower binding signals, supporting strong antibody specificity and minimal cross-reactivity. Z-scores represent the strength of antibody binding signals measured in standard deviations above the mean signal across the array, while S-scores represent the difference between ranked Z-scores and therefore reflect the relative target specificity of an antibody for its intended antigen.

SDS-PAGE analysis of purified, BSA-free StAR antibody (clone STAR/2154) as confirmation of integrity and purity.