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Home >> Antibodies >> Sp5 Antibody

Sp5 Antibody (R32265)

  Catalog No Formulation Size Price (USD)  
Image R32265 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Western blot testing of human MCF7 cell lysate with Sp5 antibody. Expected/observed molecular weight ~42 kDa.
IHC testing of FFPE human placenta with Sp5 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q6BEB4
Localization Nuclear
Applications Western blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This Sp5 antibody is available for research use only.
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Description

Sp5 is mapped to 2q31.1. It is a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Elevated expression of Sp5 has been noted in several human tumors including hepatocellular carcinoma, gastric cancer and colon cancer.

Application Notes

Optimal dilution of the Sp5 antibody should be determined by the researcher.

Immunogen

Amino acids DFAQYQSQIAALLQTKAPLAATARRCRRCR of human Sp5 were used as the immunogen for the Sp5 antibody.

Storage

After reconstitution, the Sp5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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