SOX9 Antibody / SRY-box transcription factor 9 [clone PCRP-SOX9-1A2] (V8969)

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX9 antibody (clone PCRP-SOX9-1A2). These results demonstrate the foremost specificity of the PCRP-SOX9-1A2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Immunohistochemistry analysis of SOX9 antibody in human pancreatic tissue. FFPE human pancreas sections show distinct HRP-DAB brown nuclear staining in ductal epithelial cells, consistent with nuclear localization of SOX9 as a transcription factor. Acinar cells demonstrate minimal staining, while scattered stromal cells are largely negative. The staining pattern highlights SOX9 expression within pancreatic ductal structures and progenitor-associated epithelial compartments. Heat induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, by boiling for 20 minutes followed by cooling prior to antibody incubation.

SDS-PAGE analysis of purified, BSA-free SOX9 antibody (clone PCRP-SOX9-1A2) as confirmation of integrity and purity.