SRY Box 4 Antibody SOX4/2540 / SOX4 Monoclonal Antibody [clone SOX4/2540] (V3874)

SRY Box 4 Antibody SOX4/2540 by IHC in human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human colon carcinoma tissue using SRY Box 4 Antibody clone SOX4/2540. Brown immunoreactivity is visible in scattered cells and along portions of the glandular epithelial structures, with prominent staining in individual nuclei. The staining pattern is heterogeneous, with numerous surrounding cells exhibiting little detectable signal. Hematoxylin counterstaining highlights nuclei in blue. The SOX4 Monoclonal Antibody was applied following heat-induced epitope retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6, for 10-20 minutes, followed by cooling.

SRY Box 4 Antibody SOX4/2540 by IHC in human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human breast carcinoma tissue using SRY Box 4 Antibody clone SOX4/2540. Brown immunoreactivity is prominent in groups of carcinoma cells, with staining involving both nuclear and cytoplasmic regions. The distribution is heterogeneous, with strongly stained tumor cell clusters adjacent to areas exhibiting comparatively weak staining. Surrounding connective tissue shows limited immunoreactivity. Hematoxylin counterstaining provides blue nuclear contrast. The SOX4 Monoclonal Antibody was applied following heat-induced epitope retrieval in 10 mM citrate buffer, pH 6, for 10-20 minutes, followed by cooling.

SRY Box 4 Antibody SOX4/2540 by protein microarray. Protein Microarray Validated analysis of SRY Box 4 Antibody clone SOX4/2540 using a HuProt human protein microarray containing more than 19,000 full-length proteins. The graph shows a prominent binding signal for SOX4, with additional reactivity toward SOX12, another member of the SOXC transcription factor family. Most other proteins represented in the analysis exhibit substantially lower signals. Although the SOX4 Monoclonal Antibody recognizes its intended target, the observed SOX12 binding indicates that its specificity is not exclusive to SOX4. This cross-reactivity should be considered when interpreting results from samples expressing both proteins.

SRY Box 4 Antibody SOX4/2540 purity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free SRY Box 4 Antibody clone SOX4/2540 under non-reduced (NR) and reduced (R) conditions. The non-reduced preparation shows a prominent band near 150 kDa, consistent with intact immunoglobulin. The reduced preparation displays bands corresponding to immunoglobulin heavy and light chains, migrating at approximately 50 kDa and 25 kDa, respectively. Molecular weight standards are included for reference. This analysis evaluates antibody integrity and purity rather than the detection of endogenous SOX4 protein. The SOX4 Monoclonal Antibody was purified using Protein G affinity chromatography.