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Home >> Antibodies >> SOX18 Antibody

SOX18 Antibody (RQ6340)

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  Catalog No Formulation Size Price (USD)  
Image RQ6340 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HepG2 cells with SOX18 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HepG2 cells with SOX18 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human Jurkat, 2) human COLO-320, 3) human HeLa, 4) human HepG2, 5) monkey COS-7, 6) rat heart, 7) rat lung, 8) rat liver, 9) mouse lung and 10) mouse liver lysate with SOX18 antibody. Predicted molecular weight ~41 kDa.
Flow cytometry testing of human HepG2 cells with SOX18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SOX18 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P35713
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cellsDirect ELISA
Limitations This SOX18 antibody is available for research use only.
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Description

Transcription factor SOX-18 is a protein that in humans is encoded by the SOX18 gene. This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein plays a role in hair, blood vessel, and lymphatic vessel development. Mutations in this gene have been associated with recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia.

Application Notes

Optimal dilution of the SOX18 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E63-E362) was used as the immunogen for the SOX18 antibody.

Storage

After reconstitution, the SOX18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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