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Home >> Antibodies >> Smooth Muscle Actin Antibody

Smooth Muscle Actin Antibody [clone SMAT2-1] (V7150)

  Catalog No Formulation Size Price (USD)  
Image V7150-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V7150-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V7150SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
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V7150IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 459
IHC testing of FFPE human angiosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human leiomyosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat lung with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of permeabilized human HeLa cells with Smooth Muscle Actin antibody (green, clone SMAT2-1) and Reddot nuclear stain (red).
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name SMAT2-1
Purity Protein G affinity chromatography
UniProt P62736
Localization Cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Smooth Muscle Actin antibody is available for research use only.
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Description

Actins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq]

Application Notes

Optimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody.

Storage

Store the Smooth Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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