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Home >> Antibodies >> SMAD3 Antibody

SMAD3 Antibody (F52046)

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  Catalog No Formulation Size Price (USD)  
Image F52046-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F52046-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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SMAD3 antibody western blot analysis in HepG2 and mouse C2C12 lysate. Observed molecular weight: 48~55 kDa.
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SMAD3 antibody western blot analysis in HepG2 and mouse C2C12 lysate. Observed molecular weight: 48~55 kDa.
SMAD3 antibody IHC analysis in formalin fixed and paraffin embedded brain tissue.
Confocal immunofluorescent analysis of SMAD3 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
SMAD3 antibody flow cytometric analysis of MDA-MB231 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
  • SMAD3 antibody western blot analysis in HepG2 and mouse C2C12 lysate. Observed molecular weight: 48~55 kDa.
  • SMAD3 antibody IHC analysis in formalin fixed and paraffin embedded brain tissue.
  • Confocal immunofluorescent analysis of SMAD3 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
  • SMAD3 antibody flow cytometric analysis of MDA-MB231 cells (green) compared to a <a href=negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." title="SMAD3 antibody flow cytometric analysis of MDA-MB231 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." onClick="showhideiamges('image4','imagelargedata4',4,4)">
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Rat, Pig, Chicken
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P84022
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This SMAD3 antibody is available for research use only.
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Description

SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Application Notes

Titration of the SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 186-215 from the human protein was used as the immunogen for this SMAD3 antibody.

Storage

Aliquot the SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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