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Home >> Antibodies >> SM-MHC Antibody

SM-MHC Antibody [clone ID8] (V2750)

  Catalog No Formulation Size Price (USD)  
Image V2750-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V2750-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V2750SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V2750IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
IHC: Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with SM-MHC antibody (ID8).
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with SM-MHC antibody (ID8).
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ID8
Purity Protein G affinity chromatography
UniProt P35749
Localization Cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This SM-MHC antibody is available for research use only.
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Description

SM-MHC, or Myosin-11, is a smooth muscle myosin belonging to the myosin heavy chain family. Myosin-11 is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It is a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. [Wiki]

Application Notes

Optimal dilution of the SM-MHC antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human uterus extract was used as the immunogen for the SM-MHC antibody.

Storage

Store the SM-MHC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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