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Home >> Antibodies >> SHROOM2 Antibody / APXL

SHROOM2 Antibody / APXL (RQ7652)

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  Catalog No Formulation Size Price (USD)  
Image RQ7652 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U-2 OS cells with SHROOM2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SHROOM2 antibody.
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Flow cytometry testing of human U-2 OS cells with SHROOM2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SHROOM2 antibody.
Western blot testing of human 1) Caco-2 and 2) MCF7 cell lysate with SHROOM2 antibody. Predicted molecular weight ~176 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q13796
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This SHROOM2 antibody is available for research use only.
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Description

Shroom family member 2 is a protein that in humans is encoded by the SHROOM2 gene. This gene represents the human homolog of Xenopus laevis apical protein (APX) gene, which is implicated in amiloride-sensitive sodium channel activity. It is expressed in endothelial cells and facilitates the formation of a contractile network within endothelial cells. Depletion of this gene results in an increase in endothelial sprouting, migration, and angiogenesis. This gene is highly expressed in the retina, and is a strong candidate for ocular albinism type 1 syndrome. Alternatively spliced transcript variants have been found for this gene.

Application Notes

Optimal dilution of the SHROOM2 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R427-H1138) was used as the immunogen for the SHROOM2 antibody.

Storage

After reconstitution, the SHROOM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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