SGK1 Antibody / Serine/Threonine Protein Kinase Antibody (R31780)

SGK1 Antibody HeLa Cell IF. Immunofluorescent staining of HeLa cells was performed with SGK1 Antibody / Serine/Threonine Protein Kinase Antibody (red) following enzyme-mediated antigen retrieval for 15 minutes, with DAPI used as a nuclear counterstain (blue). The image demonstrates predominantly cytoplasmic staining with perinuclear enrichment and relatively little nuclear signal, consistent with the known intracellular localization of SGK1 under basal conditions. This SGK1 Antibody supports immunofluorescence studies of serine/threonine protein kinase signaling, cellular stress responses, and cancer cell biology.

SGK1 Antibody Human Cancer Cell WB. Western blot analysis of SGK1 Antibody / Serine/Threonine Protein Kinase Antibody detected a prominent band at approximately 59 kDa in human cancer cell lysates, compared with the predicted molecular weight of approximately 49 kDa. Lanes: (1) human HeLa, (2) human SiHa, and (3) human K562 lysates. The upward mobility shift is consistent with post-translational modification of SGK1, particularly phosphorylation, which is known to alter its apparent molecular weight during SDS-PAGE. This SGK1 Antibody supports western blot studies of serine/threonine protein kinase signaling and SGK1 activation in human cancer cell models.