SDHB Antibody / Complex II Iron-Sulfur Subunit Antibody (RQ4182)

SDHB Antibody Mouse and Rat Heart and Kidney WB. Western blot analysis was performed using SDHB Antibody on rat heart and kidney tissue lysates (lanes 1-2) and mouse heart and kidney tissue lysates (lanes 3-4). Samples (30 ug) were separated by 10% SDS-PAGE under reducing conditions. The antibody was used at 0.5 ug/ml overnight at 4oC. A prominent band was detected at approximately 29 kDa, close to the predicted molecular weight of approximately 32 kDa. This SDHB Antibody demonstrates detection of the Complex II iron-sulfur subunit in mitochondria-rich mouse and rat tissues.

SDHB Antibody Human, Mouse and Rat WB. Western blot analysis was performed using SDHB Antibody on human HepG2, 293T, HeLa and THP-1 cell lysates (lanes 1-4), rat heart and brain tissue lysates (lanes 5-6), and mouse heart and brain tissue lysates (lanes 7-8). Samples (30 ug) were separated by 13% SDS-PAGE under reducing conditions. The antibody was used at 0.5 ug/ml overnight at 4oC. A specific band was detected at approximately 29 kDa, close to the predicted molecular weight of approximately 32 kDa. This SDHB Antibody demonstrates detection of the Complex II iron-sulfur subunit across human, mouse and rat samples.

SDHB Antibody Human Colon Cancer IHC. IHC staining of FFPE human colon cancer tissue was performed with SDHB Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive SDHB staining is observed in the tumor cells with a predominantly cytoplasmic pattern. This SDHB Antibody demonstrates localization of the Complex II iron-sulfur subunit in human colon cancer tissue.

SDHB Antibody Human Lung Cancer IHC. IHC staining of FFPE human lung cancer tissue was performed with SDHB Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive SDHB staining is observed in tumor cells with a predominantly cytoplasmic pattern. This SDHB Antibody demonstrates localization of the Complex II iron-sulfur subunit in human lung cancer tissue.

SDHB Antibody Human Breast Cancer IHC. IHC staining of FFPE human breast cancer tissue was performed with SDHB Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive SDHB staining is observed in tumor cells with a predominantly cytoplasmic pattern. This SDHB Antibody demonstrates localization of the Complex II iron-sulfur subunit in human breast cancer tissue.

SDHB Antibody Mouse Small Intestine IHC. IHC staining of FFPE mouse small intestine tissue was performed with SDHB Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive SDHB staining is observed prominently in the intestinal epithelium with a predominantly cytoplasmic pattern. This SDHB Antibody demonstrates localization of the Complex II iron-sulfur subunit in mouse small intestine tissue.

SDHB Antibody Rat Small Intestine IHC. IHC staining of FFPE rat small intestine tissue was performed with SDHB Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive SDHB staining is observed prominently in the intestinal epithelium with a predominantly cytoplasmic pattern. This SDHB Antibody demonstrates localization of the Complex II iron-sulfur subunit in rat small intestine tissue.