S100A16 Antibody / Tumor Progression and Cell Proliferation Marker [clone S100A16/7412] (V4720)

S100A16 Antibody Proliferative Epidermis IHC. Immunohistochemistry of S100 calcium binding protein A16 / S100A16 in FFPE human skin tissue using mouse monoclonal S100A16 antibody, clone S100A16/7412. HRP-DAB brown staining highlights prominent cytoplasmic and membranous labeling of keratinocytes within the epidermis, with increased intensity in basal and suprabasal layers consistent with proliferative and tumor-associated epithelial signaling, while the underlying dermis shows minimal staining and nuclei are counterstained blue. Inset: PBS was used in place of primary antibody as a negative control to confirm specificity of staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

S100A16 Antibody Intestinal Proliferation IHC. Immunohistochemistry of S100 calcium binding protein A16 / S100A16 in FFPE human duodenum tissue using mouse monoclonal S100A16 antibody, clone S100A16/7412. HRP-DAB brown staining highlights strong cytoplasmic and apical membranous labeling of enterocytes lining villous structures, with prominent signal along the epithelial layer consistent with active cellular turnover and proliferation-associated expression, while the lamina propria shows minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

S100A16 Antibody Tumor Epithelium IHC. Immunohistochemistry of S100 calcium binding protein A16 / S100A16 in FFPE human colon carcinoma tissue using mouse monoclonal S100A16 antibody, clone S100A16/7412. HRP-DAB brown staining highlights strong cytoplasmic and membranous labeling of malignant epithelial cells forming glandular structures, with heterogeneous intensity consistent with tumor-associated proliferation and altered differentiation, while surrounding stromal elements show minimal staining and nuclei are counterstained blue. Inset: PBS was used in place of primary antibody as a negative control to confirm specificity of staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

S100A16 Antibody Proliferation Marker WB. Western blot analysis of S100 calcium binding protein A16 / S100A16 in human SK-BR-3 cell lysate using S100A16 antibody, clone S100A16/7412. A band is detected at approximately 10-12 kDa, consistent with the predicted molecular weight of S100A16, supporting detection of this calcium-binding protein in proliferative epithelial-derived tumor cells.

SDS-PAGE analysis of purified, BSA-free S100A16 antibody (clone S100A16/7412) as confirmation of integrity and purity.