RPL13A Antibody / 60S Ribosomal Protein L13a Antibody (RQ5609)

RPL13A Antibody IHC in Human Ovarian Cancer. Immunohistochemistry of FFPE human ovarian cancer tissue was performed with RPL13A Antibody at 2 ug/ml following heat-induced epitope retrieval in pH 8 EDTA buffer. Widespread brown staining is visible throughout the tumor tissue, with prominent cytoplasmic labeling in many cells and variable staining intensity across the section.

RPL13A Antibody IHC in Rat Spleen. Immunohistochemistry of FFPE rat spleen tissue was performed with RPL13A Antibody at 2 ug/ml following heat-induced epitope retrieval in pH 8 EDTA buffer. Numerous cells demonstrate brown cytoplasmic staining, with heterogeneous signal intensity among the densely distributed splenic cell populations.

RPL13A Antibody Immunofluorescence in A549 Cells. Human A549 cells were stained with RPL13A Antibody at 5 ug/ml (green) and counterstained with DAPI (blue) following enzyme-mediated antigen retrieval. Fluorescence is distributed predominantly throughout the cytoplasm and perinuclear region, with comparatively little signal within the DAPI-positive nuclei. The staining demonstrates intracellular detection of RPL13A in A549 cells.

Western blot testing of human 1) placenta, 2) HEK293, 3) A549, 4) HepG2 and 5) K562 lysate with RPL13A antibody. Predicted molecular weight ~23 kDa.

RPL13A Antibody Western Blot in Human, Rat and Mouse Samples. Western blot testing of human RT4 (lane 1), HEL (lane 2), 293T (lane 3), and THP-1 (lane 4) cell lysates, rat spleen (lane 5) and thymus (lane 6), and mouse spleen (lane 7) and thymus (lane 8) tissue lysates was performed with RPL13A Antibody at 0.5 ug/ml. A predominant band is detected at approximately 24 kDa across all eight samples, consistent with the expected molecular weight of RPL13A. Signal is substantially stronger in the human cell lysates than in the rat and mouse tissues.

RPL13A Antibody Immunoprecipitation in RT4 Cells. Immunoprecipitation of RPL13A from human RT4 whole cell lysate was performed with 2 ug of RPL13A Antibody. Western blot analysis shows enrichment of a distinct RPL13A band at approximately 24 kDa in the antibody IP lane, consistent with the expected molecular weight. No corresponding RPL13A band is visible in the control IgG lane, while the prominent band near 50-55 kDa represents IgG heavy chain. This result demonstrates specific enrichment of RPL13A from RT4 cell lysate.