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Home >> Antibodies >> RPB2 Antibody / POLR2B

RPB2 Antibody / POLR2B (RQ8383)

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  Catalog No Formulation Size Price (USD)  
Image RQ8383 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human A549 cells with RPB2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with RPB2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human A431, 2) human HeLa, 3) human Ramos, 4) human U-251, 5) rat brain, 6) rat RH35, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with RPB2 antibody. Predicted molecular weight ~134 kDa.
Flow cytometry testing of fixed and permeabilized human U-251 cells with RPB2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPB2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P30876
Localization Nuclear
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This RPB2 antibody is available for research use only.
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Description

DNA-directed RNA polymerase II subunit RPB2 is an enzyme that in humans is encoded by the POLR2B gene. This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the RPB2 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (E49-Q1145) was used as the immunogen for the RPB2 antibody.

Storage

After reconstitution, the RPB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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