TP53 Antibody N-Terminus / p53 N-Terminal Antibody [clone rBP53-12] (V3647)

TP53 Antibody N-Terminus / p53 N-Terminal Antibody (clone rBP53-12). Protein microarray specificity validation using a HuProt(TM) human protein array containing more than 19,000 full-length human proteins demonstrates selective binding of the recombinant monoclonal clone rBP53-12. The ranked signal plot shows TP53 as the top detected target, supporting the specificity of the TP53 Antibody N-Terminus for the p53 protein.
The Z-score represents the strength of antibody binding to each protein target detected using a fluorescently labeled secondary antibody. Z-scores are expressed in standard deviations above the mean signal across the array. Proteins are ranked by descending Z-score, and the S-score represents the difference between adjacent Z-scores in the ranked list. A high S-score indicates strong specificity of the antibody for its intended target relative to other proteins present on the array.

TP53 Antibody N-Terminus / p53 N-Terminal Antibody (clone rBP53-12). Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human colon carcinoma demonstrates nuclear staining of tumor epithelial cells using the recombinant mouse monoclonal clone rBP53-12. The HRP-DAB brown chromogenic signal highlights nuclear localization of Tumor protein p53 / TP53 within malignant glandular epithelial cells, while surrounding stromal elements show minimal staining. Tissue sections were subjected to heat-induced epitope retrieval by boiling in pH 6.0, 10mM citrate buffer for 10-20 minutes followed by cooling at room temperature prior to antibody incubation.

SDS-PAGE analysis of purified, BSA-free recombinant TP53 antibody (clone rBP53-12) as confirmation of integrity and purity.

TP53 Antibody N-Terminus / p53 N-Terminal Antibody (clone rBP53-12). Western blot analysis of human HeLa cell lysate demonstrates detection of Tumor protein p53 using the recombinant mouse monoclonal clone rBP53-12. Lane 1: human HeLa cell lysate. A band is detected at approximately 53 kDa, consistent with the predicted molecular weight of Tumor protein p53 / TP53. This result confirms that the TP53 Antibody N-Terminus detects endogenous p53 protein in human cell lysates by western blot analysis.