- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
TOP2A Antibody / Cell Proliferation Marker Antibody [clone rTOP2A/8339] (V4483)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4483-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4483-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4483SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rTOP2A/8339 |
| Purity | Protein A/G affinity |
| UniProt | P11388 |
| Localization | Nucleus |
| Applications | Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 minutes at RT |
| Limitations | This recombinant TOP2A antibody is available for research use only. |
|
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DNA topoisomerase II alpha (TOP2A) is a nuclear enzyme that regulates DNA topology during replication, transcription, and chromosome segregation. The protein belongs to the type II DNA topoisomerase family and introduces transient double strand DNA breaks that relieve torsional stress generated during DNA replication and chromatin condensation. Because TOP2A expression rises sharply during the S phase and G2/M phases of the cell cycle, the enzyme is strongly associated with actively dividing cells and proliferating cell populations.
TOP2A antibody, also known as Topoisomerase II alpha antibody or DNA Topoisomerase II alpha antibody, recognizes a nuclear enzyme that functions as a well established cell proliferation marker. During cell cycle progression, TOP2A accumulates in nuclei of cells undergoing DNA replication and mitosis. For this reason the protein is widely used as a marker of proliferating cells in biological research, particularly in tissues or experimental systems where rapid cell division occurs.
TOP2A Antibody / Cell Proliferation Marker Antibody (clone rTOP2A/8339) is a recombinant mouse monoclonal antibody designed to detect this proliferation associated nuclear protein. As a Cell Proliferation Marker Antibody, clone rTOP2A/8339 enables identification of cells actively progressing through the cell cycle. Recombinant monoclonal antibodies provide consistent binding performance and reproducible recognition of the target protein across experiments, supporting reliable detection of proliferating cell populations.
The designation Cell Proliferation Marker Antibody differentiates this antibody page from other TOP2A antibody pages that focus on enzymatic terminology such as Topoisomerase II alpha antibody or DNA Topoisomerase II alpha antibody. While those pages emphasize the biochemical role of the enzyme in DNA topology regulation, this page highlights the biological significance of TOP2A as a marker of proliferating cells during cell cycle progression. This differentiation allows researchers searching specifically for a cell proliferation marker antibody to identify antibodies targeting TOP2A based on its biological function rather than only its enzyme name.
TOP2A expression is especially prominent in tissues containing rapidly dividing cells. Lymphoid germinal centers, developing epithelial compartments, and many tumor tissues display strong expression of this DNA replication enzyme because these environments contain actively proliferating cells. In lymphoid tissues such as tonsil or lymph node, proliferating B lymphocytes within germinal centers often show strong nuclear expression of TOP2A, reflecting intense DNA replication activity during immune responses.
The Cell Proliferation Marker Antibody clone rTOP2A/8339 therefore provides a valuable reagent for identifying proliferating cells in experimental systems where cell cycle activity is being studied. By detecting the TOP2A protein associated with DNA replication and mitotic chromosome segregation, this antibody supports research investigating cell proliferation, tumor growth, and cell cycle regulation in biological samples.
Optimal dilution of the TOP2A Antibody / Cell Proliferation Marker Antibody should be determined by the researcher.
A recombinant human Topoisomerase II alpha protein fragment (aa1300-1500) was used as the immunogen for the TOP2A Antibody / Cell Proliferation Marker Antibody.
Aliquot the recombinant TOP2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Topoisomerase II alpha antibody, DNA Topoisomerase II alpha antibody, DNA topoisomerase II antibody, Topoisomerase IIa antibody, TOP2A nuclear enzyme antibody
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