TNFSF15 Antibody / VEGI Cytokine Antibody [clone VEGI/2052R] (V3636)

TNFSF15 Antibody Protein Microarray Specificity Analysis. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant rabbit monoclonal clone VEGI/2052R identified TNFSF15 as the dominant target with a Z-score of 70.29 and an S-score of 55.85, supporting highly selective recognition of VEGI / TNFSF15 relative to unrelated proteins present on the array. The elevated S-score demonstrates strong preferential binding to TNFSF15 with limited off-target reactivity. The Z-score represents signal intensity generated by antibody binding relative to the mean signal across the array, while the S-score reflects the difference in signal strength between successive ranked targets and serves as a measure of relative target specificity.

TNFSF15 Antibody Parathyroid Mass IHC. Immunohistochemistry staining of FFPE human parathyroid mass tissue using recombinant rabbit monoclonal clone VEGI/2052R demonstrates diffuse cytoplasmic HRP-DAB brown staining in neoplastic epithelial cells, consistent with expression of TNFSF15 / VEGI within endocrine-associated cellular populations. The observed staining pattern supports the involvement of this TNF superfamily cytokine in inflammatory and tissue regulatory signaling pathways. Required HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at room temperature for 20 min.

TNFSF15 Antibody Colon Carcinoma IHC. Immunohistochemistry staining of FFPE human colon carcinoma tissue using recombinant rabbit monoclonal clone VEGI/2052R demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within gland-forming malignant epithelial cells, consistent with expression of TNFSF15 / VEGI in epithelial and inflammatory signaling-associated tissue compartments. The observed staining pattern supports the involvement of this TNF superfamily cytokine in tumor-associated immune regulation and vascular signaling pathways. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at room temperature for 20 min prior to staining.

TNFSF15 Antibody Spleen IHC. Immunohistochemistry staining of FFPE human spleen tissue using recombinant rabbit monoclonal clone VEGI/2052R demonstrates membranous and cytoplasmic HRP-DAB brown staining predominantly within vascular endothelial structures and scattered stromal-associated cells, consistent with expression of TNFSF15 / VEGI in vascular and immune-regulatory tissue compartments. The observed staining pattern supports the established role of this TNF superfamily cytokine in inflammatory signaling, endothelial biology, and immune pathway regulation. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at room temperature for 20 min prior to staining.

SDS-PAGE analysis of purified, BSA-free recombinant TNFSF15 antibody (clone VEGI/2052R) as confirmation of integrity and purity.