TNFSF15 Antibody Microarray Validated / TL1A / VEGI [clone VEGI/2052R] (V3636)

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant TNFSF15 antibody (clone VEGI/2052R). These results demonstrate the foremost specificity of the VEGI/2052R mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human parathyroid mass with recombinant TNFSF15 antibody (clone VEGI/2052R). Required HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Immunohistochemistry of TNFSF15 antibody in human colon carcinoma tissue. FFPE human colon carcinoma demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within gland-forming tumor epithelial cells, consistent with VEGI / TNFSF15 localization in epithelial and vascular-associated compartments. Clone VEGI/2052R was used as a recombinant rabbit monoclonal antibody that is Microarray Validated. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to staining.

Immunohistochemistry of TNFSF15 antibody in human spleen tissue. FFPE human spleen demonstrates membranous and cytoplasmic HRP-DAB brown staining predominantly within vascular endothelial structures and scattered stromal cells, consistent with VEGI / TNFSF15 expression in vascular and immune-associated compartments. Clone VEGI/2052R was used as a recombinant rabbit monoclonal antibody that is Microarray Validated. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to staining.

SDS-PAGE analysis of purified, BSA-free recombinant TNFSF15 antibody (clone VEGI/2052R) as confirmation of integrity and purity.