Rabbit IgG Fc Antibody for ELISA / Biotinylated Anti-Rabbit IgG Fc Detection Antibody [clone RMG02] (R20173BTN)

Rabbit IgG Fc Antibody Biotin ELISA Species Specificity Analysis. ELISA analysis of IgG from multiple species demonstrates that the parent clone RMG02 antibody selectively recognizes rabbit IgG and rabbit IgG Fc, with strong signal observed across tested concentrations, while no detectable binding is observed with human, mouse, or rat IgG. This binding profile reflects Fc-region-specific recognition and strict species selectivity and is representative of the performance retained in the biotinylated format of the Rabbit IgG Fc Antibody for ELISA / Biotinylated Anti-Rabbit IgG Fc Detection Antibody.

Rabbit IgG Fc Antibody Biotin WB Analysis. Western blot analysis of rabbit IgG under non-reducing (-) and reducing (+) conditions demonstrates that the parent clone RMG02 antibody detects a band at approximately 150 kDa in the non-reduced sample, consistent with intact rabbit IgG. No significant signal is observed under reducing conditions, supporting Fc-region-dependent recognition that is sensitive to structural disruption. Lane 1: non-reduced rabbit IgG, Lane 2: reduced rabbit IgG. This binding profile reflects Fc-specific recognition of native IgG and is representative of the performance retained in the biotinylated format of the Rabbit IgG Fc Antibody / Biotinylated Anti-Rabbit IgG Fc Detection Antibody.

Rabbit IgG Fc Antibody Biotin ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of rabbit IgG demonstrates strong, concentration-dependent binding of clone RMG02 across a broad dynamic range. Signal intensity decreases proportionally with both antigen concentration and antibody dilution, confirming consistent Fc-region-specific recognition of rabbit IgG. Detection was performed using an alkaline phosphatase-conjugated anti-goat IgG secondary antibody. This titration profile reflects the intrinsic binding performance of the parent clone RMG02 antibody and is representative of the characteristics retained in the biotinylated format of the Rabbit IgG Fc Antibody for ELISA / Biotinylated Anti-Rabbit IgG Fc Detection Antibody.