- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant Poly His Antibody / His Tag [clone 6HIS/6402R] (V9221)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V9221-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V9221-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V9221SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Species independent |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | 6HIS/6402R |
| Purity | Protein A/G affinity |
| Applications | Western Blot : 1-2ug/ml Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml |
| Limitations | This recombinant Poly His antibody is available for research use only. |
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Recombinant Poly His antibody recognizes the polyhistidine tag, commonly referred to as His-tag or 6xHis tag, a short amino acid sequence widely used for recombinant protein purification and detection. Poly His antibody, also referred to as anti-His antibody and His tag antibody in the literature, detects histidine-tagged fusion proteins expressed in bacterial, mammalian, or insect systems. Clone 6HIS/6402R is produced as a recombinant rabbit monoclonal antibody, providing defined specificity and lot-to-lot consistency for detection of His-tagged proteins in research applications.
The polyhistidine tag typically consists of six consecutive histidine residues engineered at the N- or C-terminus of recombinant proteins. This sequence enables affinity purification using immobilized metal affinity chromatography and allows straightforward immunodetection in downstream assays. Because the His-tag is small and generally does not interfere with protein folding or function, it is one of the most widely used affinity tags in molecular biology.
Detection of His-tagged proteins is essential for verifying expression, confirming purification, and assessing protein integrity. In denatured lysates, intact fusion proteins are typically detected at their expected molecular weight, while truncated or degraded forms may also be observed depending on expression system and stability. Reliable recognition of the polyhistidine epitope is therefore important for accurate interpretation of recombinant protein experiments.
Clone 6HIS/6402R recognizes the polyhistidine sequence independent of the fused protein backbone, supporting broad detection across diverse recombinant constructs. A recombinant rabbit monoclonal format supports consistent performance across production lots and enables reproducible detection of His-tagged targets in research workflows.
Recombinant Poly His antibody is suitable for research applications focused on detection, characterization, and analysis of His-tagged recombinant proteins in expression and purification systems.
Optimal dilution of the recombinant Poly His antibody should be determined by the researcher.
Hexa-histidine tagged human protein was used as the immunogen for the recombinant Poly His antibody.
Aliquot the recombinant Poly His antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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