Recombinant PD-L1 Antibody [clone PDL1/4280R] (V8640)

IHC staining of FFPE human placenta with recombinant PD-L1 antibody (clone PDL1/4280R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung small cell carcinoma with recombinant PD-L1 antibody (clone PDL1/4280R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant PD-L1 antibody (clone PDL1/4280R) as confirmation of integrity and purity.

Recombinant PD-L1 Antibody in Multi-Species Lung WB. Western blot testing of Recombinant PD-L1 Antibody in lung tissue lysates from human, mouse, rat, hamster, guinea pig and rabbit. A prominent band is detected in the human lung sample at approximately 45 kDa, consistent with the extensively glycosylated mature form of PD-L1/CD274. Little to no corresponding signal is observed in the other species under the tested conditions. This Recombinant PD-L1 Antibody demonstrates strong detection of human PD-L1 with minimal cross-reactivity in the tested non-human lung samples.

Recombinant PD-L1 Antibody in Human Lung WB. Western blot testing of Recombinant PD-L1 Antibody in human lung tissue lysate. A prominent band is observed at approximately 45 kDa, above the predicted molecular weight of ~33 kDa for unmodified PD-L1/CD274. This higher apparent molecular weight is consistent with the extensive N-linked glycosylation of mature PD-L1, which commonly produces a glycosylated protein migrating at approximately 45-55 kDa. This Recombinant PD-L1 Antibody demonstrates detection of the mature glycosylated form of PD-L1 in human lung tissue.