TP53 Antibody for Western Blot / p53 Western Blot Antibody [clone rTP53/1739] (V3939)

TP53 Antibody for Western Blot / p53 Western Blot Antibody (clone rTP53/1739). Western blot analysis of human HeLa cell lysate demonstrates detection of Tumor protein p53 using the recombinant mouse monoclonal clone rTP53/1739. Lane 1: human HeLa cell lysate. A band is detected at approximately 53 kDa, consistent with the predicted molecular weight of Tumor protein p53 / TP53. This result confirms that the TP53 Antibody for Western Blot detects endogenous p53 protein in human cell lysates by western blot analysis.

TP53 Antibody for Western Blot / p53 Western Blot Antibody (clone rTP53/1739). Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast carcinoma demonstrates nuclear staining of tumor epithelial cells using the recombinant mouse monoclonal clone rTP53/1739. The chromogenic HRP-DAB brown signal highlights strong nuclear localization of Tumor protein p53 / TP53 in malignant cells, while surrounding stromal elements show minimal staining. Tissue sections were subjected to heat-induced epitope retrieval by boiling in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature prior to antibody incubation.

TP53 Antibody for Western Blot / p53 Western Blot Antibody (clone rTP53/1739). Protein microarray specificity validation using a HuProt(TM) human protein array containing more than 19,000 full-length human proteins demonstrates selective binding of the recombinant monoclonal clone rTP53/1739. The ranked signal plot shows TP53 as the top detected target, supporting strong specificity of the TP53 Antibody for Western Blot for Tumor protein p53.
The Z-score represents the strength of antibody binding to each protein target detected using a fluorescently labeled secondary antibody. Z-scores are expressed in standard deviations above the mean signal across the array. Proteins are ranked by descending Z-score, and the S-score represents the difference between adjacent Z-scores in the ranked list. A high S-score indicates strong specificity of the antibody for its intended target relative to other proteins present on the array.

SDS-PAGE analysis of purified, BSA-free recombinant p53/TP53 antibody (clone rTP53/1739) as confirmation of integrity and purity.