p53 Antibody Microarray Specificity Validated Clone TP53/2092R / TP53 Tumor Suppressor Antibody [clone TP53/2092R] (V3646)

p53 Antibody Microarray Specificity Validated Clone TP53/2092R / TP53 Tumor Suppressor Antibody. Western blot analysis of human HeLa cell lysate demonstrates detection of Tumor protein p53 using the recombinant rabbit monoclonal clone TP53/2092R. Lane 1: human HeLa cell lysate. A band is detected at approximately 53 kDa, consistent with the predicted molecular weight of Tumor protein p53 / TP53. This result supports specific recognition of endogenous p53 protein by the Microarray Specificity Validated clone TP53/2092R in western blot analysis.

p53 Antibody Microarray Specificity Validated Clone TP53/2092R / TP53 Tumor Suppressor Antibody. Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human colon carcinoma demonstrates strong nuclear staining of tumor epithelial cells using the recombinant rabbit monoclonal clone TP53/2092R. The HRP-DAB brown chromogenic signal highlights nuclear localization of Tumor protein p53 / TP53 within malignant glandular epithelial cells, while surrounding stromal elements show minimal staining. Tissue sections were subjected to heat-induced epitope retrieval by boiling in pH 6.0, 10mM citrate buffer for 10-20 minutes followed by cooling at room temperature prior to antibody incubation.

p53 Antibody Microarray Specificity Validated Clone TP53/2092R / TP53 Tumor Suppressor Antibody. Protein microarray specificity validation using a HuProt(TM) human protein array containing more than 19,000 full-length human proteins demonstrates highly selective binding of the recombinant rabbit monoclonal clone TP53/2092R. The ranked signal plot shows TP53 as the top detected target, supporting the specificity of the p53 Antibody Microarray Specificity Validated clone TP53/2092R for Tumor protein p53.
The Z-score represents the strength of antibody binding to each protein target detected using a fluorescently labeled secondary antibody. Z-scores are expressed in standard deviations above the mean signal across the array. Proteins are ranked by descending Z-score, and the S-score represents the difference between adjacent Z-scores in the ranked list. A high S-score indicates strong specificity of the antibody for its intended target relative to other proteins present on the array.

SDS-PAGE analysis of purified, BSA-free p53 antibody (clone TP53/2092R) as confirmation of integrity and purity.