Recombinant p27Kip1 Antibody / p27 / CDKN1B [clone KIP1/1355R] (V9526)

p27Kip1 Antibody Human Bladder Tissue IHC. Immunohistochemistry staining of FFPE human bladder tissue with recombinant p27Kip1 antibody (clone clone KIP1/1355R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. Strong nuclear staining observed.

SDS-PAGE analysis of purified, BSA-free recombinant p27Kip1 antibody (clone KIP1/1355R) as confirmation of integrity and purity.

Western blot testing of human A431 cell lysate with recombinant p27Kip1 antibody. Expected molecular weight ~27 kDa.

Western blot testing of mouse NIH 3T3 cell lysate with recombinant p27Kip1 antibody. Expected molecular weight ~27 kDa.

p27Kip1 Antibody Multi-Species Skeletal Muscle WB. Western blot analysis of p27Kip1 / CDKN1B expression across skeletal muscle lysates from multiple species using p27Kip1 antibody clone KIP1/1355R. Lane 1: human skeletal muscle lysate, Lane 2: mouse skeletal muscle lysate, Lane 3: rat skeletal muscle lysate, Lane 4: hamster skeletal muscle lysate, Lane 5: guinea pig skeletal muscle lysate, Lane 6: rabbit skeletal muscle lysate. A band is detected at approximately 27-29 kDa in all samples, consistent with the predicted molecular weight of p27Kip1 (CDKN1B), a cyclin-dependent kinase inhibitor that regulates cell cycle progression. The consistent signal across species supports the use of this clone for cross-species western blot analysis.

p27Kip1 Antibody Human Tissue WB. Western blot analysis of p27Kip1 / CDKN1B expression in human tissue lysates using p27Kip1 antibody clone KIP1/1355R. Lane 1: human spleen lysate, Lane 2: human skeletal muscle lysate. A band is detected at approximately 27-29 kDa in both samples, consistent with the predicted molecular weight of p27Kip1 (CDKN1B), a cyclin-dependent kinase inhibitor that regulates cell cycle progression. Detection across distinct tissue types supports the use of this clone for western blot analysis in diverse human samples.