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Home >> Antibodies >> Recombinant p21 Antibody / CDKN1A

Recombinant p21 Antibody / CDKN1A [clone CIP1/2489R] (V7655)

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  Catalog No Formulation Size Price (USD)  
Image V7655-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7655-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7655SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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p21 Antibody Human Lung and HeLa WB. Western blot analysis of p21 / CDKN1A, also known as p21WAF1, expression in human lung tissue and HeLa cell lysates using p21 antibody clone CIP1/2489R. Lane 1: human lung lysate, Lane 2: HeLa cell lysate. A band is detected at approximately 21-24 kDa, consistent with the predicted molecular weight of p21 (CDKN1A), a key cyclin-dependent kinase inhibitor that regulates cell cycle arrest. Signal is observed in both samples, reflecting p21 expression in tissue and proliferating cells.
SDS-PAGE analysis of purified, BSA-free recombinant p21 antibody (clone CIP1/2489R) as confirmation of integrity and purity.
IHC staining of FFPE human urothelial carcinoma with recombinant p21 antibody (clone CIP1/2489R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CIP1/2489R
Purity Protein A affinity chromatography
UniProt P38936
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This recombinant p21 antibody is available for research use only.
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Description

This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

This antibody is part of a broader antibody panel offered by NSJ Bioreagents.

Application Notes

Optimal dilution of the recombinant p21 antibody should be determined by the researcher.

Immunogen

A recombinant full-length human protein was used as the immunogen for the recombinant p21 antibody.

Storage

Store the recombinant p21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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