Recombinant Nuclear Antigen Antibody for WB / Western Blot Validated Antibody [clone rNM106] (V8854)

Recombinant Nuclear Antigen Antibody for WB Multi-Species Western Blot. Western blot analysis of heart, liver, and skeletal muscle tissue lysates from human, mouse, rat, hamster, guinea pig, and rabbit using Recombinant Nuclear Antigen Antibody for WB / Western Blot Validated Antibody (clone rNM106) demonstrates a strong immunoreactive band at approximately 110-116 kDa across all tested tissues and species. The highly consistent banding pattern indicates broad cross-species reactivity and recognition of a conserved nuclear-associated antigen expressed in diverse mammalian cell types. Robust signal is observed in cardiac, hepatic, and skeletal muscle samples from each species tested, supporting reliable detection of the target antigen in multiple biological systems. Because the recognized nuclear antigen is widely expressed and highly conserved, clone rNM106 serves as a valuable reagent for comparative biology studies, translational research, species cross-reactivity assessment, and western blot applications requiring broad mammalian reactivity. The observed molecular weight remains consistent across all tested samples and demonstrates the utility of this recombinant antibody for detection of nuclear-associated proteins in mammalian tissue lysates. Observed molecular weight: ~110-116 kDa.

Recombinant Nuclear Antigen Antibody for WB Human Tissue Western Blot. Western blot analysis of human heart, intestine, liver, and skeletal muscle tissue lysates using Recombinant Nuclear Antigen Antibody for WB / Western Blot Validated Antibody (clone rNM106) demonstrates a strong immunoreactive band at approximately 110-116 kDa in all tissues examined. The highly consistent banding pattern indicates robust recognition of a broadly expressed nuclear-associated antigen across multiple human tissue types. Comparable signal intensity is observed in cardiac, gastrointestinal, hepatic, and skeletal muscle samples, supporting widespread expression of the target antigen and reliable antibody performance in diverse biological specimens. Because the recognized nuclear antigen is conserved and broadly distributed among mammalian cell types, clone rNM106 serves as a valuable reagent for western blot studies involving tissue characterization, nuclear-associated protein detection, and comparative expression analysis. The observed molecular weight is consistent across all tested human tissues and demonstrates the utility of this recombinant antibody for detection of nuclear-associated antigens by western blot. Observed molecular weight: ~110-116 kDa.

Recombinant Nuclear Antigen Antibody Human Tonsil Tissue IHC. Immunohistochemistry staining of FFPE human tonsil tissue using Pan-Nuclear Antigen Antibody (clone rNM106) demonstrates strong nuclear HRP-DAB brown staining throughout lymphoid cell populations. The staining pattern is consistent with recognition of a broadly expressed nuclear-associated antigen present within the nuclei of diverse cell types. Distinct nuclear localization provides clear visualization of cellular distribution, tissue architecture, and nuclear morphology while minimizing background staining in non-nuclear compartments. As a pan-nuclear marker, clone rNM106 is useful for identification of cell nuclei, assessment of tissue organization, and subcellular localization studies in both normal and pathological specimens. The observed staining highlights widespread nuclear labeling throughout the tonsillar lymphoid compartment and demonstrates the utility of this recombinant antibody for nuclear visualization applications.

SDS-PAGE analysis of purified, BSA-free recombinant Nuclear Antigen antibody (clone rNM106) as confirmation of integrity and purity.