MYH11 Antibody / Smooth Muscle Contractile Protein Antibody [clone rMYH11/8066] (V4421)

MYH11 Antibody Cross-Species Gastrointestinal WB. Western blot analysis of intestine and stomach tissue lysates from multiple species was performed using MYH11 antibody clone rMYH11/8066. Samples include human, mouse, rat, hamster, guinea pig, and rabbit intestine and stomach tissues. MYH11, also known as Smooth Muscle Myosin Heavy Chain, is a major component of the contractile apparatus and a highly specific marker of differentiated smooth muscle cells. Strong bands are detected at approximately 200 kDa in human, guinea pig, and rabbit intestine, consistent with the expected molecular weight of full length MYH11. Minor higher molecular weight species likely represent alternatively spliced isoforms or post-translationally modified forms. These results demonstrate the cross-species reactivity of recombinant MYH11 antibody and support its utility for studies of smooth muscle differentiation, gastrointestinal physiology, and comparative biology.

MYH11 Antibody Human Gastrointestinal Tissues WB. Western blot analysis of human intestine and human stomach tissue lysates was performed using MYH11 antibody clone rMYH11/8066. MYH11, also known as Smooth Muscle Myosin Heavy Chain, is a major contractile protein and a highly specific marker of differentiated smooth muscle cells. Prominent immunoreactive bands are detected at approximately 200 kDa in both tissues, corresponding to the expected molecular weight of full-length MYH11. The broader signal observed in intestine likely reflects the presence of multiple MYH11 isoforms and post-translationally modified species associated with visceral smooth muscle. These results support the utility of recombinant MYH11 antibody for studies of smooth muscle differentiation, gastrointestinal physiology, and contractile function.

MYH11 Antibody Colon Smooth Muscle IHC. Immunohistochemical staining of FFPE human colon smooth muscle tissue demonstrates MYH11 expression using recombinant MYH11 antibody clone rMYH11/8066. MYH11, also known as Smooth Muscle Myosin Heavy Chain, is a principal component of the contractile apparatus and a highly specific marker of differentiated smooth muscle cells. Diffuse cytoplasmic staining is observed throughout bundles of smooth muscle fibers within the muscularis propria, consistent with the abundant expression of MYH11 in visceral smooth muscle responsible for intestinal motility. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining. These results support the utility of MYH11 Antibody for studies of smooth muscle differentiation, gastrointestinal physiology, and contractile function.

SDS-PAGE analysis of purified, BSA-free recombinant MYH11 antibody (clone rMYH11/8066) as confirmation of integrity and purity.