IHC staining of FFPE human colon carcinoma tissue with recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue from a patient with Lynch syndrome using recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human HCT-116 cell lysate using recombinant MSH6 antibody (clone MSH6/6654R). Expected molecular weight: 120-160 kDa depending on phosphorylation level.
Recombinant MSH6 Antibody MSH6/6654R in Human Esophagus IHC. Immunohistochemistry testing of Recombinant MSH6 Antibody, clone MSH6/6654R, in FFPE human esophagus tissue. Strong nuclear staining is observed in epithelial cells, consistent with the nuclear localization of MSH6. The inset shows a secondary-only negative control in which PBS was substituted for the primary antibody, demonstrating minimal background staining under the tested conditions.
Recombinant MSH6 Antibody MSH6/6654R in Dog Lymph Node IHC. Immunohistochemistry testing of Recombinant MSH6 Antibody, clone MSH6/6654R, in FFPE dog lymph node tissue. Heat-induced epitope retrieval was performed in Tris/EDTA buffer, pH 9.0, for 45 min. An HRP-polymer secondary detection system was applied for 30 min followed by DAB for 5 min. Nuclear staining is observed in a subset of lymphoid cells, consistent with the nuclear localization of MSH6.
Recombinant MSH6 Antibody MSH6/6654R in Cat Colon IHC. Immunohistochemistry testing of Recombinant MSH6 Antibody, clone MSH6/6654R, in FFPE cat colon tissue. Heat-induced epitope retrieval was performed in Tris/EDTA buffer, pH 9.0, for 45 min. An HRP-polymer secondary detection system was applied for 30 min followed by DAB for 5 min. Nuclear staining is observed in colonic epithelial cells, consistent with the nuclear localization of MSH6.