• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Recombinant MSH6 Antibody

Recombinant MSH6 Antibody [clone MSH6/6654R] (V9182)

  Catalog No Formulation Size Price (USD)  
Image V9182-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
Image
V9182-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
Image
V9182SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
Bulk quote request
IHC staining of FFPE human colon carcinoma tissue with recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue from a patient with Lynch syndrome using recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human HCT-116 cell lysate using recombinant MSH6 antibody (clone MSH6/6654R). Expected molecular weight: 120-160 kDa depending on phosphorylation level.
Recombinant MSH6 Antibody MSH6/6654R in Human Esophagus IHC. Immunohistochemistry testing of Recombinant MSH6 Antibody, clone MSH6/6654R, in FFPE human esophagus tissue. Strong nuclear staining is observed in epithelial cells, consistent with the nuclear localization of MSH6. The inset shows a secondary-only negative control in which PBS was substituted for the primary antibody, demonstrating minimal background staining under the tested conditions.
Recombinant MSH6 Antibody MSH6/6654R in Dog Lymph Node IHC. Immunohistochemistry testing of Recombinant MSH6 Antibody, clone MSH6/6654R, in FFPE dog lymph node tissue. Heat-induced epitope retrieval was performed in Tris/EDTA buffer, pH 9.0, for 45 min. An HRP-polymer secondary detection system was applied for 30 min followed by DAB for 5 min. Nuclear staining is observed in a subset of lymphoid cells, consistent with the nuclear localization of MSH6.
Recombinant MSH6 Antibody MSH6/6654R in Cat Colon IHC. Immunohistochemistry testing of Recombinant MSH6 Antibody, clone MSH6/6654R, in FFPE cat colon tissue. Heat-induced epitope retrieval was performed in Tris/EDTA buffer, pH 9.0, for 45 min. An HRP-polymer secondary detection system was applied for 30 min followed by DAB for 5 min. Nuclear staining is observed in colonic epithelial cells, consistent with the nuclear localization of MSH6.
Availability 1-3 business days
Species Reactivity Human, Dog, Cat
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name MSH6/6654R
Purity Protein A/G affinity
UniProt P52701
Localization Nuclear
Applications ELISA (order BSA-free Format For Coating) :
Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant MSH6 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H).

For a protein microarray-validated reagent and additional information on mismatch repair and genomic stability, visit our MSH6 Antibody / DNA Mismatch Repair Protein Antibody page.

Application Notes

Optimal dilution of the recombinant MSH6 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1-100 was used as the immunogen for the recombinant MSH6 antibody.

Storage

Aliquot the recombinant MSH6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.