Mouse IgG2b Antibody for ELISA / Anti-Mouse IgG2b ELISA Detection Antibody [clone RM108] (R20167)

Mouse IgG2b Antibody for WB. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG2b (20 ng per lane) using clone RM108 at 0.2 ug/mL demonstrates strong detection of intact IgG2b at approximately 150 kDa under non-reducing conditions, with a weaker band near 50 kDa corresponding to the heavy chain under reducing conditions. The stronger signal observed in the non-reduced sample is consistent with recognition of native immunoglobulin structure and supports selective detection of mouse IgG2b. These results support the use of this Mouse IgG2b Antibody / Anti-Mouse IgG2b Detection Antibody for reliable detection in immunoassay applications.

Mouse IgG2b Antibody for ELISA Subclass Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that clone RM108 selectively recognizes Mouse IgG2b, with strong signal observed across tested concentrations. No cross-reactivity is detected with IgG1, IgG2a, IgG3, IgM, IgA, or IgE, or with human, rat, or goat IgG. The binding profile is consistent with Fc region-directed recognition and supports selective detection of IgG2b independent of light chain composition. These results support the use of this Mouse IgG2b Antibody for ELISA / Anti-Mouse IgG2b ELISA Detection Antibody for precise subclass-specific detection in ELISA-based immunoassays.

Mouse IgG2b Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of mouse IgG2b demonstrates strong, concentration-dependent binding of clone RM108 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding performance. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody, supporting reliable quantification of Mouse IgG2b in ELISA-based immunoassays.