Mouse IgG2b Antibody for ELISA / Anti-Mouse IgG2b ELISA Detection Antibody [clone RM108] (R20167)
 Catalog No.  Formulation  Size
R20167-100UG
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
100 ug
Bulk quote request
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM108
Purity Protein A purified from animal origin-free supernatant
UniProt P01867
Gene ID 16016
Applications ELISA : 0.005ug/ml-0.2ug/ml
Western Blot (stronger On Non-reduced Samples) : 0.1-0.5ug/ml
Limitations This Mouse IgG2b Antibody for ELISA / Anti-Mouse IgG2b ELISA Detection Antibody is available for research use only.
Mouse IgG2b Antibody for WB. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG2b (20 ng per lane) using clone RM108 at 0.2 ug/mL demonstrates strong detection of intact IgG2b at approximately 150 kDa under non-reducing conditions, with a weaker band near 50 kDa corresponding to the heavy chain under reducing conditions. The stronger signal observed in the non-reduced sample is consistent with recognition of native immunoglobulin structure and supports selective detection of mouse IgG2b. These results support the use of this Mouse IgG2b Antibody / Anti-Mouse IgG2b Detection Antibody for reliable detection in immunoassay applications.
Mouse IgG2b Antibody for WB. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG2b (20 ng per lane) using clone RM108 at 0.2 ug/mL demonstrates strong detection of intact IgG2b at approximately 150 kDa under non-reducing conditions, with a weaker band near 50 kDa corresponding to the heavy chain under reducing conditions. The stronger signal observed in the non-reduced sample is consistent with recognition of native immunoglobulin structure and supports selective detection of mouse IgG2b. These results support the use of this Mouse IgG2b Antibody / Anti-Mouse IgG2b Detection Antibody for reliable detection in immunoassay applications.