Mouse IgG2b Antibody for ELISA / Biotinylated Anti-Mouse IgG2b ELISA Detection Antibody [clone RM108] (R20167BTN)

Mouse IgG2b Antibody Biotin WB. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG2b demonstrates that the parent clone RM108 antibody detects intact IgG2b at approximately 150 kDa under non-reducing conditions, with weaker detection of the reduced heavy chain near 50 kDa. This binding pattern reflects recognition of native immunoglobulin structure and confirms specificity for mouse IgG2b. These results represent the intrinsic binding characteristics of clone RM108 and are retained in the biotinylated format of the Mouse IgG2b Antibody / Biotinylated Anti-Mouse IgG2b Detection Antibody.

Mouse IgG2b Antibody Biotin ELISA Subclass Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that the parent clone RM108 antibody selectively recognizes Mouse IgG2b, with strong signal observed across tested concentrations. No cross-reactivity is detected with IgG1, IgG2a, IgG3, IgM, IgA, or IgE, or with human, rat, or goat IgG. This binding profile reflects Fc region-directed specificity for mouse IgG2b and is retained in the biotinylated format of the Mouse IgG2b Antibody for ELISA / Biotinylated Anti-Mouse IgG2b ELISA Detection Antibody.

Mouse IgG2b Antibody Biotin ELISA Titration Curve. ELISA titration using plates coated with serial dilutions of mouse IgG2b demonstrates strong, concentration-dependent binding of clone RM108 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding kinetics. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. This titration profile reflects the intrinsic binding performance of the parent clone RM108 antibody and is representative of the characteristics retained in the biotinylated format of the Mouse IgG2b Antibody for ELISA / Biotinylated Anti-Mouse IgG2b ELISA Detection Antibody.