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Home >> Antibodies >> Recombinant MART-1 Antibody / Melan-A

Recombinant MART-1 Antibody / Melan-A [clone MLANA/4475R] (V8661)

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  Catalog No Formulation Size Price (USD)  
Image V8661-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V8661-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V8661SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Western blot testing of human COLO-38 cell lysate with recombinant MART-1 antibody (clone MLANA/4475R). Expected molecular weight ~20 kDa with possible doublet.
IHC staining of FFPE human skin with recombinant MART-1 antibody (clone MLANA/4475R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin with recombinant MART-1 antibody (clone MLANA/4475R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name MLANA/4475R
Purity Protein A affinity chromatography
UniProt Q16655
Localization Cytoplasmic
Applications Western Blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant MART-1 antibody is available for research use only.
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Description

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Explore our Melan-A Antibody / Melanocyte Lineage Marker clone A103 page for additional validation data and research applications involving melanoma biology, melanocytic tumor identification, and pigment cell differentiation pathways.

Application Notes

Optimal dilution of the recombinant MART-1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human MART-1 protein was used as the immunogen for the recombinant MART-1 antibody.

Storage

Store the recombinant MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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