Keratin 10 Antibody rKRT10/1275 / Recombinant CK10 Monoclonal Antibody [clone rKRT10/1275] (V3602)

Keratin 10 Antibody rKRT10/1275 by WB in Human Skin. Western blot analysis of human skin tissue lysate using Keratin 10 Antibody rKRT10/1275. A prominent immunoreactive band is detected at approximately 56-60 kDa, consistent with the expected molecular weight of cytokeratin 10 (56.5 kDa). The single sample lane shows a strong, relatively well-defined band with minimal additional immunoreactivity. The Recombinant CK10 Monoclonal Antibody demonstrates detection of endogenous KRT10 in human skin, where this intermediate filament protein is expressed predominantly in differentiated suprabasal keratinocytes. The observed migration is consistent with the predicted size of the target protein.

Keratin 10 Antibody rKRT10/1275 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Keratin 10 Antibody rKRT10/1275 under non-reducing (NR) and reducing (R) conditions, with 2.5 ug of antibody loaded per lane. The non-reducing lane shows a prominent band near 150 kDa, consistent with intact IgG. Under reducing conditions, distinct bands are observed at approximately 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. The Recombinant CK10 Monoclonal Antibody demonstrates the expected electrophoretic profile of a purified mouse monoclonal antibody preparation.

Keratin 10 Antibody rKRT10/1275 by IHC in Human Skin. Immunohistochemistry of FFPE human skin tissue using Keratin 10 Antibody rKRT10/1275. Strong brown cytoplasmic staining is observed in differentiated suprabasal keratinocytes, while the basal epidermal layer exhibits comparatively weak staining. The Recombinant CK10 Monoclonal Antibody highlights the expected distribution of KRT10 in stratified squamous epithelium. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.