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Home >> Antibodies >> Recombinant IgM Heavy Chain Antibody

Recombinant IgM Heavy Chain Antibody [clone rIM373] (V7425)

  Catalog No Formulation Size Price (USD)  
Image V7425-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V7425-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V7425SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
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V7425IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 459
IHC staining of FFPE human tonsil tissue with recombinant IgM Heavy Chain antibody (clone rIM373). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant IgM Heavy Chain antibody (clone rIM373) as confirmation of integrity and purity.

Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rIM373
Purity Protein G affinity chromatography
Gene ID 3507
Localization Cytoplasm, cell surface and secreted
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant IgM Heavy Chain antibody is available for research use only.
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Description

This antibody recognizes a protein of 75kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Heavy Chain antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant protein was used as the immunogen for this recombinant IgM Heavy Chain antibody.

Storage

Store the recombinant IgM Heavy Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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