Recombinant IBA1 Antibody - Microarray Validated / AIF1 [clone rAIF1/1909] (V3831)

Immunohistochemistry of Recombinant IBA1 Antibody Microarray Validated clone rAIF1/1909 in human lymph node tissue. Formalin-fixed, paraffin-embedded lymph node demonstrates cytoplasmic HRP-DAB brown staining in numerous macrophage-lineage cells within the paracortical and sinusoidal regions, consistent with Allograft inflammatory factor 1 expression in tissue macrophages and activated immune cells. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6, 10mM citrate buffer for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to staining. The recombinant monoclonal antibody clone rAIF1/1909 was used as the primary antibody.

Immunohistochemistry of Recombinant IBA1 Antibody Microarray Validated clone rAIF1/1909 in human tonsil tissue. Formalin-fixed, paraffin-embedded tonsil demonstrates cytoplasmic HRP-DAB brown staining in scattered macrophage-lineage cells within the lymphoid parenchyma and interfollicular regions, consistent with Allograft inflammatory factor 1 expression in tissue macrophages and activated immune cells. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6, 10mM citrate buffer for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to staining. The recombinant monoclonal antibody clone rAIF1/1909 was used as the primary antibody.

Human protein microarray specificity validation of Recombinant IBA1 Antibody Microarray Validated clone rAIF1/1909. Analysis was performed using the HuProt(TM) microarray containing more than 19,000 full-length human proteins. The antibody demonstrates the highest signal intensity for AIF1, ranking first among all proteins tested with a strong Z-score and clear separation from lower ranked proteins, supporting high target specificity of clone rAIF1/1909.
Z- and S-score explanation: The Z-score represents the strength of signal generated when the antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a specific protein on the HuProt(TM) array. Z-scores are expressed in units of standard deviations above the mean signal of all proteins on the array. Proteins are ranked in descending order according to Z-score. The S-score represents the difference in Z-scores between adjacent ranked proteins and reflects the relative specificity of the antibody for its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant IBA1 antibody (clone rAIF1/1909) as confirmation of integrity and purity.