Human IgD Antibody for ELISA / Immunoglobulin D ELISA Antibody [clone RM123] (R20185)

Human IgD Antibody for ELISA sandwich ELISA detection of human IgD in plasma. Plates were coated with Human IgD Antibody for ELISA (clone RM123) at 100 ng per well as the capture antibody. Human plasma samples containing IgD were applied in serial dilutions, and bound immunoglobulin was detected using biotinylated anti-human light chains (kappa+lambda) antibody clone RM129, followed by alkaline phosphatase conjugated streptavidin. The assay demonstrates sensitive detection of human IgD present in plasma samples.

Human IgD Antibody for ELISA specificity analysis against human immunoglobulins. ELISA of human immunoglobulins demonstrates that Human IgD Antibody for ELISA (clone RM123) reacts with IgD derived from human plasma and IgD,kappa from human myeloma. No cross reactivity is observed with other immunoglobulin classes including IgG, IgM, IgA, or IgE under the tested conditions.

Human IgD Antibody for ELISA titration analysis against human IgD. ELISA titration was performed using plates coated with varying amounts of human IgD derived from plasma (50 uL per well). A serial dilution of Human IgD Antibody for ELISA (clone RM123) was applied as the primary antibody, followed by detection with an alkaline phosphatase conjugated anti-rabbit IgG secondary antibody. The results demonstrate concentration-dependent binding of the antibody to human IgD across the tested coating concentrations.