Human IgA2 Antibody for ELISA / Anti-IgA2 Detection Antibody [clone RM125] (R20184)

Human IgA2 Antibody for ELISA Sandwich ELISA Human Plasma. Sandwich ELISA analysis demonstrates that clone RM125 functions effectively as a capture antibody for Human IgA2 / IGHA2, with signal intensity decreasing proportionally with plasma dilution, indicating strong and concentration-dependent detection. Captured IgA2 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. This assay supports reliable detection of IgA2 in plasma and highlights the utility of this Human IgA2 Antibody for ELISA / Anti-IgA2 Detection Antibody for subclass-specific ELISA-based immunoassays.

Human IgA2 Antibody for ELISA Sandwich ELISA Human IgA2. Sandwich ELISA analysis using purified human IgA2 demonstrates that clone RM125 functions effectively as a capture antibody for Human IgA2 / IGHA2, with signal intensity decreasing proportionally with antigen concentration, indicating strong and concentration-dependent detection. Captured IgA2 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. This assay configuration supports accurate quantification of IgA2 and highlights the utility of this Human IgA2 Antibody for ELISA / Anti-IgA2 Detection Antibody in subclass-specific ELISA-based immunoassays.

Human IgA2 Antibody Immunohistochemistry Human Tonsil Tissue. Immunohistochemistry analysis of FFPE human tonsil tissue using Human IgA2 Antibody / Anti-IgA2 Detection Antibody (clone RM125) demonstrates cytoplasmic staining in plasma cells localized predominantly within interfollicular and subepithelial regions, while germinal center lymphocytes show minimal staining. The staining pattern supports detection of IgA2 / IGHA2 in mucosal-associated antibody-secreting cells and is consistent with IgA2 enrichment in barrier-associated immune compartments. Heat-induced epitope retrieval was performed using pH6 citrate buffer or pH9 Tris-EDTA buffer prior to antibody incubation.

Human IgA2 Antibody for ELISA Subclass Specificity Analysis. ELISA analysis of human immunoglobulins demonstrates that clone RM125 selectively recognizes Human IgA2 / IGHA2, with strong signal observed across tested concentrations, indicating specific binding to the alpha 2 heavy chain. No detectable cross-reactivity is observed with IgA1 or other immunoglobulin classes including IgG1, IgG2, IgG3, IgG4, IgM, IgD, or IgE. This binding profile supports highly selective IgA2 detection and highlights the utility of this Human IgA2 Antibody for ELISA / Anti-IgA2 Detection Antibody for subclass-specific ELISA-based immunoassays.

Human IgA2 Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of human IgA2 demonstrates strong, concentration-dependent binding of clone RM125 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution and antigen concentration, confirming high sensitivity and consistent binding performance. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody, supporting reliable quantification of Human IgA2 / IGHA2 in ELISA-based immunoassays.