Human IgA2 Antibody for ELISA Clone RM125

	Catalog No	Formulation	Size	Price (USD)
	R20184-100UG	1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide	100 ug	439
Formulation

Sandwich ELISA of human plasma using recombinant Human IgA2 antibody as the capture (100ng/well), and biotinylated anti-human light chains (Îº+Î») antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

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Sandwich ELISA of human plasma using recombinant Human IgA2 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

Sandwich ELISA of human IgA2 using recombinant Human IgA2 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

IHC testing of FFPE human tonsil with recombinant Human IgA2 antibody (clone RM125). A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Human IgA2 Antibody for ELISA Clone RM125 specificity by ELISA. ELISA analysis demonstrates the specificity of Recombinant Human IgA2 Antibody RM125 against purified human immunoglobulins coated at 50 ng per well. The antibody shows strong reactivity with human IgA2 across multiple concentrations (200 ng/mL, 50 ng/mL, and 10 ng/mL), while no detectable binding is observed with human IgA1, IgG subclasses (IgG1, IgG2, IgG3, IgG4), IgM, IgD, or IgE. These results indicate that Recombinant Human IgA2 Antibody RM125 selectively recognizes the Immunoglobulin alpha 2 heavy chain (IGHA2) and does not cross react with other immunoglobulin classes or subclasses.

Human IgA2 Antibody for ELISA clone RM125 titration by ELISA. ELISA titration analysis of Human IgA2 Antibody for ELISA clone RM125 using plates coated with varying amounts of purified human IgA2 (50 uL per well coating). A serial dilution of the recombinant rabbit monoclonal antibody RM125 was applied as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG secondary antibody for detection. The assay shows concentration-dependent binding to human IgA2, with higher absorbance values observed at increased coating concentrations and higher primary antibody concentrations. These results demonstrate specific recognition of the Immunoglobulin alpha 2 heavy chain (IGHA2) by clone RM125 in ELISA-based detection assays.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Rabbit
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG
Clone Name	RM125
Purity	Protein A purified from animal origin-free supernatant
UniProt	P01877
Gene ID	3494
Applications	ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection) Immunocytochemistry : 0.5-2ug/ml Immunohistochemistry : 0.1-1ug/ml
Limitations	This Human IgA2 antibody is available for research use only.
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Description

Immunoglobulin alpha 2 heavy chain (IGHA2) is the heavy chain that defines the IgA2 subclass of immunoglobulin A antibodies, an important component of humoral immunity at mucosal surfaces. IgA antibodies are produced by differentiated B cells and plasma cells and function as a major protective mechanism against pathogens encountered at epithelial barriers. The Recombinant Human IgA2 Antibody for ELISA Clone RM125 recognizes the IgA2 subclass of human immunoglobulin A and enables specific detection of IgA2-containing antibodies in biological samples. IgA2 is particularly prominent in mucosal tissues where exposure to microbial antigens is frequent and immune defense must be maintained without excessive inflammation.

IgA antibodies occur as two subclasses in humans, IgA1 and IgA2, which differ in structural features of their hinge regions and their susceptibility to bacterial proteases. IgA2 has a shorter hinge region compared with IgA1, making it more resistant to proteolytic cleavage by microbial enzymes that are common in mucosal environments. This structural adaptation contributes to the enrichment of IgA2 in intestinal secretions and other mucosal compartments. The IGHA2 gene encodes the alpha 2 heavy chain responsible for these subclass-specific structural characteristics.

IgA2 antibodies are generated by plasma cells located within mucosa-associated lymphoid tissues such as Peyer's patches, intestinal lamina propria, tonsils, and other lymphoid organs involved in mucosal immunity. These antibodies contribute to immune exclusion by binding microbial antigens and preventing pathogen attachment to epithelial surfaces. Secretory IgA complexes containing IgA2 are transported across epithelial cells via the polymeric immunoglobulin receptor and released into mucosal secretions where they participate in the neutralization of pathogens and maintenance of mucosal immune homeostasis.

Because IgA subclass composition can vary among tissues and immune responses, an IgA2 antibody provides a useful tool for investigating subclass-specific humoral immunity. Detection of Immunoglobulin alpha 2 heavy chain expression allows researchers to evaluate IgA2-producing plasma cells and examine the distribution of IgA subclasses within mucosal and lymphoid tissues. Understanding IgA2 expression patterns can also contribute to studies of host-microbe interactions, mucosal immune regulation, and antibody-mediated defense mechanisms in human immune responses.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Recombinant Human IgA2 Antibody for ELISA Clone RM125 may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human IgA2 was used as the immunogen for this recombinant Human IgA2 antibody.

Storage

Store the recombinant Human IgA2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Alternate Names

IgA2 antibody, Immunoglobulin A2 antibody, IGHA2 antibody, IgA subclass 2 antibody, Ig alpha heavy chain 2 antibody

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Human IgA2 Antibody for ELISA Clone RM125 / IGHA2 [clone RM125] (R20184)

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