HSP60 Antibody / Heat Shock Protein 60 Antibody [clone rGROEL/780] (V3576)

HSP60 Antibody / Heat Shock Protein 60 Antibody Multi-Species Tissue Expression WB. Western blot testing of brain, liver and pancreas tissue lysates from human, mouse, rat, hamster, guinea pig and rabbit with HSP60 Antibody clone rGROEL/780 shows a strong predominant band at approximately 60 kDa across all tested samples. The observed band is consistent with the expected molecular weight of HSP60 and demonstrates recognition across multiple mammalian species and tissue types.

HSP60 Antibody / Heat Shock Protein 60 Antibody in HeLa Cervical Cancer Cells WB. Western blot testing of human HeLa cervical cancer cell lysate with HSP60 Antibody clone rGROEL/780 shows a strong, specific band at approximately 60 kDa. The observed band is consistent with the predicted molecular weight of HSPD1 and demonstrates detection of HSP60 in this human cervical cancer cell line.

Protein Microarray Validated HSP60 Antibody Specificity. HuProt protein microarray analysis of HSP60 Antibody clone rGROEL/780 against more than 19,000 full-length human proteins identifies HSPD1 as the highest-ranked target, with a Z-score of 128.43 and S-score of 116.3. The Z-score represents signal strength in standard deviations above the mean array signal, while the S-score represents the difference between the intended target Z-score and the next highest signal. The substantial separation of HSPD1 from the next-ranked proteins demonstrates strong relative target specificity.

Purified HSP60 Antibody rGROEL/780 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free HSP60 Antibody clone rGROEL/780 under non-reduced (NR) and reduced (R) conditions. The non-reduced antibody migrates predominantly at approximately 90 kDa, while reduction produces prominent heavy- and light-chain bands at approximately 50 kDa and 25-28 kDa, respectively. A 2.5 ug antibody sample was loaded for analysis, demonstrating the integrity and purity of the recombinant monoclonal preparation.

HSP60 Antibody / Heat Shock Protein 60 Antibody in Human Tissues and Cell Lines WB. Western blot testing of human brain, kidney, liver and pancreas tissue lysates and HeLa cervical cancer and Jurkat T-cell leukemia cell lysates with HSP60 Antibody clone rGROEL/780 shows a strong, specific band at approximately 60 kDa across all samples. The observed band is consistent with the expected molecular weight of HSPD1 and demonstrates robust HSP60 detection across the tested human tissues and cell lines.

HSP60 Antibody / Heat Shock Protein 60 Antibody in Human Liver IHC. Immunohistochemistry staining of FFPE human liver tissue with HSP60 Antibody clone rGROEL/780 shows granular cytoplasmic staining of hepatocytes, consistent with the mitochondrial localization of HSP60. Heat-induced epitope retrieval was performed by boiling sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.